EFFECT OF HYDROGEN PEROXIDE ON PIGMENT COMPOSITION AND CHLOROPHYLL FLUORESCENCE IN BASIL (OCIMUM BASILICUM L.) LEAVES
DOI:
https://doi.org/10.17770/etr2025vol1.8647Keywords:
basil, carotenoids, chlorophylls, hydrogen peroxide (H2O2), Ocimum basilicum L.Abstract
Basil is one of the most famous herbs, widely used fresh, and for therapeutic and pharmaceutical purposes. Basil has recently been shown to rank first among spices and herb crops regarding xanthophyll-carotenoids, which are associated with a reduced risk of cancer and age-related eye diseases. Given the widespread use of the herb basil in nutrition and herbal medicine, the contents of its leaves are of obvious interest. However, some studies say that water enriched with hydrogen peroxide helps more effectively saturate plant cells with oxygen molecules, activates the growth processes of all parts of seedlings, and increases the resistance of seedlings to various unfavorable factors, including dangerous fungal and bacterial diseases (late blight, downy mildew, root rot, powdery mildew, bacteriosis). However, in high concentrations, hydrogen peroxide also can have a phytotoxic effect on plants. The research goal for the current study was to determine the application of hydrogen peroxide (H2O2) on photosynthetic processes (the content of photosynthetic pigments, the parameters of the chlorophyll a fluorescence) in sweet basil (Ocimum basilicum L. cv. “Genovese”). The experiment was carried out under controlled conditions with three replications. Four treatments of H2O2 were tested: 0.0, 0.1, 0.5, 1.0 and 3.0%. Both photosynthetic pigments and parameters of the chlorophyll a fluorescence in basil leaves were quantified and compared with the control. The plants were cultivated in an environmental climatic chamber with conditions close to real natural conditions (temperature: 24-30°C, humidity: 70 – 80%, photoperiodism: day/night (8/16), illumination: 10 000 lux). The present results revealed the changes in chlorophyll fluorescence parameters in basil under different concentrations of H2O2 (0.1, 0.5, 1.0 3.0%). In addition, changes in photosynthetic pigment composition have also been noticed depending on the concentrations of H2O2 in the water before watering the plants. However, it is necessary to continue studying the effect of H2O2 on the whole plant in future research.
References
M.A. Aazami, M. Asghari-Aruq, M.B. Hassanpouraghdam, S. Ercisli, M. J. Baron, Sochor, “Low temperature stress mediates the antioxidants pool and chlorophyll fluorescence in Vitis vinifera L. cultivars,” Plants, vol. 10, pp. 1-12, 2021. https://doi: 10.3390/plants10091877
G.S. Araújo, S.O. Paula-Marinho, S.K.P. Pinheiro, E.C. Miguel, L.S. Lopes, E.C. Marques, H.H. Carvalho, E. Gomes-Filho, “H2O2 priming promotes salt tolerance in maize by protecting chloroplasts ultrastructure and primary metabolites modulation,” Plant Science, vol. 303, pp. 1-11, 2021. https://doi.org/10.1016/j.plantsci.2020.110774
N.S. Azizah, B. Irawan, J. Kusmoro, W. Safriansyah, K. Farabi, D. Oktavia, F. Doni, M. Miranti, “Sweet basil (Ocimum basilicum L.) – a review of its botany, phytochemistry, pharmacological activities, and biotechnological development,” Plants, vol. 12, pp. 1-19, 2023. https://doi: 10.3390/plants12244148
A. Batjuka, I. Umbraško, A. Petjukevičs, N. Škute, “Antibacterial activity of aromatic herbs aqueous extracts: oregano (Origanum vulgare L.), turmeric (Curcuma longa L.), cloves (Syzygium aromaticum L.) and coriander (Coriandrum sativum L.) on Escherichia coli ATCC 25922,”Acta Biol Univ Daugavp, vol. 2, pp. 309-320, 2024. https://doi.org/10.59893/abud.24(2).015
N.R. Baker, J. Harbinson, D.M. Kramer, “Determining the limitations and regulation of photosynthetic energy transduction in leaves,” Plant, Cell and Environment, vol. 30, pp. 1107-1125, 2007. https://doi: 10.1111/j.1365-3040.2007.01680.x
F. Balanescu, M.D.I. Mihaila, G. Cârâc, B. Furdui, C. Vỉnâtoru, S.M. Avramescu, E.L. Lisa, M. Cudalbeanu, R.M. Dinica, “Flavonoid profiles of two new approved Romanian Ocimum hybrids,” Foods, vol. 13, pp. 1-19, 2020. https://doi.org/10.3390/molecules25194573
H.C. Bravo, N.V. Céspedes, L. Zura-Bravo, L.A. Muňoz, “Basil seeds as a novel food, source of nutrients and functional ingredients with beneficial properties: a review,” Foods, vol. 10, pp. 1-18, 2021. https://doi.10.3390/foods10071467
M. Černý, H. Habánová, M. Berka, M. Luklová, B. Brzobohatý, “Hydrogen peroxide: its role in plant biology and crosstalk with signalling networks,” International Journal of Molecular Sciences, vol. 19, pp. 1-30, 2018. https://doi.org/10.3390/ijms19092812
P. Eskandarzade, M.Z. Mehrjerdi, F. Didaran, N.S. Gruda, S. Aliniaeifard, “Shading level and harvest time affect the photosynthetic and physiological properties of basil varieties,” Agronomy, vol. 13(10), pp. 1-16, 2023. https://doi: 10.20944/preprints202308.1227.v1
K. Gupta, A. Sengupta, M. Chakraborty, B. Gupta, “Hydrogen peroxide and polyamines act as double edged swords in plant abiotic stress responses,” Frontiers in Plant Sciences, vol. 7, pp. 1-19, 2016. https://doi.org/10.3389/fpls.2016.01343
H. Hassan, A. Alatawi, A. Abdulmajeed, M. Emam, H. Khattab, “Roles of Si and SiNPs improving thermotolerance of wheat photosynthetic machinery via upregulation of PsbH, PsbB and PsbD genes encoding PSII core proteins,” Horticulturae, vol. 7, pp. 1-16, 2021. https://doi.org/10.3390/horticulturae7020016
M.A. Hossain, S. Bhattacharjee, S.M. Armin, P. Qian, W. Xin, H.Y. Li, D.J. Burritt, Fujita M., L.S.P. Tran, “Hydrogen peroxide priming modulates abiotic oxidative stress tolerance: insights from ROS detoxification and scavenging,” Frontiers in Plant Science, vol. 6, pp. 1-19, 2015. https://doi.org/10.3389/fpls.2015.00420
Y. Iijima, R. Davidovich-Rikanati, E. Fridman, D.R. Gang, E. Bar, E. Lewinsohn, E. Pichersky, “The biochemical and molecular basis for the divergent patterns in the biosynthesis of terpenes and phenylpropenes in the peltate glands of three cultivars in basil,” Plant Physiology, vol. 136, pp. 3724-3736, 2004. https://doi: 10.1104/pp.104.051318
R. Jamaludin, N. Mat, K.S. Mohd, N.A. Badaluddin, K. Mahmud, M.H. Sajili, M.M. Khandaker, “Influence of exogenous hydrogen peroxide on plant physiology, leaf anatomy and rubisco gene expression of the Ficus deltoidea Jack var. Deltoidea,” Agronomy, vol. 10, pp. 1-20, 2020. https://doi.org/10.3390/agronomy10040497
T.A. Khan, M. Yusuf, Q. Fariduddin, “Seed treatment with H2O2 modifies net photosynthetic rate and antioxidant system in mung bean (Vigna radiata L. Wilczek) plants,” Israel Journal of Plant Sciences, vol. 62, pp. 167-175, 2015. https://doi.org/10.1080/07929978.2015.1060806
F. Mani, T. Bettaieb, K. Zheni, N. Doudech, C. Hannachi, “Effect of hydrogen peroxide and thiourea on fluorescence and tuberization of potato (Solanum tuberosum L.),” Journal of Stress Physiology & Biochemistry, vol. 8(3), pp. 61-71, 2012.
T. Maoka, “Carotenoids as natural functional pigments,” Journal of Natural Medicines, vol. 74, pp. 1-16, 2020. https://doi.org/10.1007/s11418-019-01364-x
E.H. Murchie, T. Lawson, “Chlorophyll fluorescence analysis: a guide to good practice and understanding some new applications,” Journal of Experimental Botany, vol. 64 (13), pp. 3983-3998, 2013. https://doi.org/10.1093/jxb/ert208
F. Nazir, Q. Fariduddin, A. Hussain, T.A. Khan, “Brassinosteroid and hydrogen peroxide improve photosynthetic machinery, stomatal movement, root morphology and cell viability and reduce cu-triggered oxidative burst in tomato,” Ecotoxicology and Environmental Safety, vol. 207, pp. 1-14, 2021. https://doi: 10.1016/j.ecoenv.2020.111081
A. Petjukevičs, A. Batjuka, N. Škute, “The impact of different levels of sodium chloride on the quantitative changes of chlorophyll and carotenoids in chloroplasts of Elodea canadensis (Michx. 1803),” Biologija, vol. 61(1), pp. 34-41, 2015. https://doi.org/10.6001/biologija.v61i1.3109
A. Petjukevics, N. Skute, “Chlorophyll fluorescence changes, as plant early state indicator under different water salinity regimes on the invasive macrophyte Elodea canadensis (Michx., 1803),” One Ecosystem, vol. 7, pp. 1-11, 2022.
https://doi.org/10.3897/oneeco.7.e82389
C. Picazo, M. Molin, “Impact of hydrogen peroxide on protein synthesis in yeast,” Antioxidants, vol. 10, pp. 1-18, 2021. https://doi: 10.3390/antiox10060952
M. Rahimi, M. Mortazavi, A. Mianabadi, S. Debnath, “Evaluation of basil (Ocimum basilicum) accessions under different drought conditions based on yield and physio-biochemical traits,” BMC Plant Biology, vol. 523, pp. 1-15, 2023. https://doi.org/10.1186/s12870-023-04554-8
M. Schrieber, N.S. Chandel, “ROS function in redox signaling and oxidative stress,” Current Biology, vol. 24(10), pp. 1-25, 2014. https://doi: 10.1016/j.cub.2014.03.034
P.C.C. Silva, H.R. Gheyi, M.J.S. Jesus, M.R.S.Correia, A.D.A. Neto, “Seed priming with hydrogen peroxide enhances tolerance to salt stress of hydroponic lettuce,” Brazilian Journal of Agricultural and Environmental Enginnering,” vol. 27(9), pp. 704-711, 2023. http://dx.doi.org/10.1590/1807-1929
M. Savicka, A. Petjukevics, A. Batjuka, N. Skute, “Impact of moderate heat stress on the biochemical and physiological responses of the invasive waterweed Elodea canadensis (Michx. 1803),” Archives of Biological Sciences, vol. 70(3), pp. 551–557, 2018. https://doi.org/10.2298/ABS180119016S
P. Swapnil, M. Meena, S.K. Singh, U.P. Dhuldhaj, A. Marwal, “Vital roles of carotenoids in plants and humans to deteriorate with its structure, biosynthesis, metabolic engineering and functional aspects,” Current Plant Biology, vol.26, pp. 1-11, 2021. https://doi.org/10.1016/j.cpb.2021.100203
I. Umbrasko, A. Batjuka, A. Petjukevics, N. Skute, “Antifungal activity of medicinal herbs aqueous extracts: common wormwood (Artemisia absinthium L.), greater burdock (Arctium lappa L.), common thyme (Thymus vulgaris L.), and pot marigold (Calendula officinalis L.) on yeast Saccharomyces cerevisiae,” Proceedings of the 15th International Scientific and Practical Conference, vol. 1, pp. 393–398, 2024. https://doi.org/10.17770/etr2024vol1.7970
E. Valanciene, I. Jonuskiene, M. Syrpas, E. Augustiniene, P. Matulis, A. Simonavicius, N. Malys, “Advances and prospects of phenolic acids production, biorefinery and analysis,” Biomolecules, vol. 10, pp. 1-41, 2020. https://doi.org/10.3390/biom10060874
S. Wang, J. Hu, B. Ren, P. Liu, B. Zhao, J. Zhang, “Effects of hydrogen peroxide priming on yield, photosynthetic capacity and chlorophyll fluorescence of waterlogged summer maize,” Frontiers in Plant Science, vol. 13, pp. 1-15, 2022. https://doi.org/10.3389/fpls.2022.1042920
A. Zhori, M. Meco, H. Brandi, R. Bachofen, “In situ chlorophyll fluorescence kinetics as a tool to quantify effects on photosynthesis in Euphorbia cyparissias by a parasitic infection on the rust fungus Uromyces pisi,” BMC Res Notes, vol. 8, pp. 1-7, 2015. https://doi.org/10.1186/s13104-015-1681-z
Downloads
Published
Issue
Section
License
Copyright (c) 2025 Anna Batjuka, Aleksandrs Petjukevics, Inta Umbrasko, Natalja Skute
This work is licensed under a Creative Commons Attribution 4.0 International License.
